Immunofluorescent analysis of Citrulline in human embryonic kidney cells (HEK293). Sample was fixed with 5% formaldehyde (5 min), incubated with Citrulline monoclonal antibody (Product # MA5-27574) using a dilution of 1:50 (30-60 min at RT), and followed by Goat Anti-Mouse Alexa Fluor 488, Phalloidin Alexa Fluor 633 and DAPI secondary antibody at a dilution of 1:1500, 1:250 and 1:50,000 (30-60 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, (B) Phalloidin Alex Fluor 633 F-Actin stain, (C) Citrulline Antibody (D) Merged image. Magnification: 20x.